Egg Donation in Prague
Why should you choose Prague Fertility Centre?
To provide comprehensive, innovative, evidence-based fertility and gynaecology treatment focused on individual problems and needs.
- 1Many years of experience in the IVF field
- 2Individual approach and high success rate
- 3Specialization in treating women over 40
- 4Guarantee of 2 transfers and convenient packages
- 5Asynchronous Embryo Transfer – ASET
- 6Advanced laboratory methods
- 7Egg donation / embryodonation with no waiting list
- 8Pleasant environment and friendly staff.
Percentage of success
Hypothetically, we have 100 patients who undergo the first fresh cycle of egg donation, 50 of these patients achieve pregnancy right after the fresh embryo transfer. Then we have another 20 pregnancies after KET (frozen embryo transfer).
So within one full cycle of egg donation we have 70 pregnant patients. Among the 30 patients who come for the second cycle another 15 of them achieve pregnancy after the fresh embryo transfer and another 6 of them after KET. Within the second full cycle of egg donation we have a cumulative pregnancy rate of around 91%.
Results of 2015
The average age of the patients: 42 years
Egg donation program
Treatment can be planned in a short period of time of about 3 months to provide comprehensive, innovative, evidence-based fertility and gynaecology treatment focused on individual problems and needs.
We use the latest ART treatments. Egg donation is our most successful program, with a success rate of about 60%. Also important in our program is our guarantee of 2 transfers.
International patients are asked to pay a deposit 3.000 Eur, this is important because the donor is prepared especially for the recipient, and her cycle is adjusted to the recipient´s, the preparation always starts a few weeks before the treatment.
IVF cycle with egg donation and guarantee includes:
To provide comprehensive, innovative, evidence-based fertility and gynaecology treatment focused on individual problems and needs.
1LAZT - Laser assisted zona thinning
Thinning a portion of the outer embryo shell by a laser for facilitating embryo’s hatching process.
On the 5th or 6th day of its development, the embryo leaves its protective outer layer (zona pellucida), which among other things has protected it till now from extracorporeal manipulation. This process of breaking out or “hatching” is necessary for the embryo to establish contact with uterus cells in which it becomes implanted.
Some embryo implantation problems in patients with recurrent implantation failure may be explained by the inability of the embryo to hatch out of its zona pellucida. This may occur naturally or hardening process can arise after vitrification. In such cases, zona pellucida can be thinned in one part using the laser technique to improve the pregnancy, implantation and delivery rates (see the list of scientific studies below). LAZT offers many advantages over traditional assisted hatching (AH).
Mainly, it does not involve opening of the protective layer and internal environment of the embryo remains intact. The risk of immunosuppressants from white cells and any local inflammatory are more harmful to the blastomeres of total zona pellucida assisted hatching. Furthermore, LAZT is not associated with an increased rate of identical monozygotic twins as opposed to zona opening methods.
There is no need of concerns about using laser since the target in reaction process is controlled accurately and has been shown to have no mechanical, thermal or mutagenic effect.
2ASET – Asynchronous embryo transfer
So called „window of implantation” (WOI) is a short period of time when the female uterus is capable of accepting a viable embryo for further development, pregnancy. Normally, the endometrium is embryo-receptive around days 19 to 21 of each menstrual cycle. In other days, or in cases of endometrial dysfunction, the female uterus is not ready for pregnancy, and healthy embryos are rejected and die (implantation failure).
As a part of infertility therapy, hormonal treatment can positively modulate the embryo-friendly changes in the female uterus before embryo transfer in IVF cycles. Moreover, the endometrium preparedness can be monitored during each menstrual cycle by ultrasound examination. However, some infertile women still fail to develop pregnancy, despite having high-quality embryos and good-looking endometrium. Endometrial dysfunction (endometrial factor) can be further tested on a molecular level by invasive Endometrial Receptivity Array (ERA test), for which a small piece of uterine mucosa is surgically biopsied and examined for function of several hundreds of genes related to embryo implantation (paid services in specialized laboratories).
In PFC, asynchronous embryo transfer (ASET) is available for patients with compromised endometrial receptivity confirmed by laboratory, as well as for patients with suspected endometrial factor. The level of endometrial receptivity may vary from cycle to cycle, and the occurrence of implantation window (WOI) may vary as well. Therefore, PFC can postpone some embryos, so that 2 or 3 embryos of different developmental stages (asynchronous) are transferred. This way, delays in WOI peak or occurrence from 1 to 5 days can be compensated for. Experts in PFC are ready to assist you in a personalized manner specific for your case.
For a limited period of time, ASET treatment is free of charge for all couples treated in PFC.
3IVF - In vitro fertilisation
During IVF eggs are placed together with washed spermatozoa and fertilization occurs spontaneously, similarly like in the oviducts of the mother. A disadvantage of this method is the low fertilization rate mainly in cases when the partner’s sperm is low-quality. Often fertilization is unsuccessful even though sperm is assessed as good-quality in semen analysis. Sometimes polyspermy occurs, when more than one sperm fertilizes an egg – the resulting embryos are abnormal (have more chromosomes than normal) and so are rejected.
4ICSI - Intracitoplasmic sperm injection
During ICSI one sperm is injected directly into the egg cytoplasm using an apparatus that transforms imperfect hand movements into fine and precise movements of micromanipulation tools. This method is used in the following cases:
Abnormal semen analysis findings ie. low sperm concentration or a high level of abnormal sperm with low motility
Fertilization using sperm surgically extracted from testicles or the epididymis (using MESA/TESE techniques)
Failure to spontaneously fertilize in the previous cycle even though semen analysis results are positive
5PICSI – ICSI with selected mature spermatozoa
In the ICSI procedure, an individual sperm is selected and injected into an oocyte. Until now, the only technique available to embryologists to select the sperm has been visual observation. Using PICSI procedure we are able to determine sperm selection in much the same way it happens in human biology.
Sperms are placed in PICSI dish containing samples of hyaluronan hydrogel. Hyaluronan is a naturally occurring biopolymer found in all human cells, including the gel layer surrounding the oocyte.
Mature, biochemically competent sperm bind to the hyaluronan where they can be isolated by the embryologist and used for ICSI. This procedure mimics a key step in the natural fertilization process, the binding of mature sperm to the oocyte complex. As a result, the selected sperm is essentially the same as one that would be successful in the natural reproductive process.
The research proved that hyaluronan-bound PICSI-selected sperm are, in the vast majority of cases, more mature, exhibit less DNA damage, and have fewer chromosomal aneuploidies.
PFC recommends PICSI method as a biologically more natural and effective form of fertilization in comparison to ICSI, because only those sperm are chosen for fertilization that are able to form a bond with the oocyte cumulus complex, i.e. only mature sperm are selected...
This method is suitable for everyone but we highly recommend it especially in the following cases:
1.) previous total failure or low fertilization even after ICSI
2.) low embryo quality or their failure to develop
3.) repeated abortions
Noninvasive time-lapse embryo imaging with detection of abnormal divisions leading to aneuploidy
Objective and non-invasive imaging of embryo characteristics allows detection of abnormal cleavages leading to the occurence of chromosomal malsegregation which are a major cause of spontaneous miscarriage or birth defects.
Scanning and analyzing embryo development is a breakthrough approach in assessing human embryos in a culture medium. It is performed by a computer which continuously monitors the embryo’s deve¬lopment. The embryos are not disturbed during their extracorporeal development by constant examinations as the entire monitoring process happens behind closed doors of the incubator. Subsequent analysis is based on acquired digital records.
The developmental abilities of human embryos can be predicted by measurement of their cell cycles. Continuous non-invasive embryo monitoring allows an exact measurement of these phases of human embryo development. The duration of the first 4 interphases and synchrony of the daughter cells cleavages are expressed in Embryo Cleavage Rating (ECR) and correspond with regular ooplasmic metabolits and organelles distribution and with embryonic genome activation (EGA).
7Magnetic-Activated Cell Sorting (MACS)
Prague Fertility Centre introduces a new generation of Magnetic-Activated Cell Sorting (MACS) system – an efficient method to select functional sperm and improve pregnancy rates.
Genetic integrity of the spermatozoon is essential for normal embryo development. A high level of DNA fragmentation in sperm cells can negatively affect embryo cleavage and subsequent development leading to blastocyst arrest or early miscarriage. DNA damage represented by fragmentation and subsequent sperm apoptosis may be a cause of male infertility that standard methods – sperm concentration, morphology assessment and motility analysis cannot detect.
A major causative factor for sperm DNA damage is oxidative stress generally increasing with the age and/or inflammatory infections, cigarette smoking, drug use, exposure to environmental pollutants and elevated testicular temperature.
Therefore, MACS Annexin V System was designed to selectively remove these defective although morphologically indistinguishable cells from sperm preparations. The procedure begins with magnetic labeling of unwanted cells and then, they are passed through a separation column where they are selectively retained. Intact living spermatozoa without DNA fragmentation pass through the column and are collected for later use (ICSI or cryopreservation).
Gain of the sperm ready for fertilization represents the key advantage over traditional DNA fragmentation tests (e.g. SCSA/SDIA, see Tab. below) in which patient obtain only impractical information on percentage of damaged cells. Similarly, methods for morphological assessing, like IMSI, cannot provide sufficient picture about the state of the DNA in the sperm nucleus.
8PGD/PGS – preimplantation genetic diagnosis
Preimplantation genetic diagnosis/ preimpl. genetic screening and ANEULOIDY PREDICTION
PGD – preimplantation genetic diagnosis is indicated in the cases of genetically transmitted diseases.
PGS – preimplantation genetic screening is indicated if meiotic errors are expected therefore, PGS is offered in the cases of advanced maternal age and severe male factor infertilities for example after TESE – surgical spermatozoa retrieval from testes
EmbryoGen® is a new advanced embryo culture medium which includes a natural communication substance used by the mother during pregnancy.
The embryo is cultured in EmbryoGen® during the IVF process and is finally returned to the woman’s uterus along with a small amount of EmbryoGen®. This way, the embryo is better prepared for dialogue when returned to the uterus.
EmbryoGen® was tested in one of the world’s largest IVF trials. Results (tested in more than 1300 IVF patients in 14 clinics) showed significant improvements in women with a history of miscarriage.
Embryo transfer is one of the most sensitive and critical procedures in IVF treatment. Implantation of embryos still presents a challenge to the clinicians since it is a multistage process which includes zona hatching, apposition and adhesion of blastocyst followed by an invasion of trophoblast into the uterine endometrium. There are several reasons why embryos fail to implant despite a favorable transfer. One of the reasons for unsuccessful implantation may be a failure to develop a sticky matrix between blastocyst and endometrium. Therefore EmbryoGlue® medium was developed exclusively for embryo transfer, helping embryos to stick to the endometrium.
EmbryoGlue® contains high concentration of hyaluronan (0.5 mg/mL) and low concentration of recombinant human albumin. It is uniquely developed to mimic the conditions in the female uterus in order to help embryos implant after transfer. Albumin, highly abundant in the female reproduction tract, serves as a source of energy and as a reservoir for the release of hormones, vitamins, and metals. Hyaluronan is present in the oviduct and uterine fluids, increases up to the time of implantation and decreases to near basal levels by the next day, indicating that it is important for implantation. It also indirectly promotes angiogenesis. By virtue of these properties, it improves apposition and attachment of embryos which are key steps in the process of implantation. It also increases viscosity, which promotes easy handling, facilitating ET process, and by virtue of apposition, it prevents expulsion of embryos from uterine cavity posttransfer.
11Vitrification method for freezing your embryos
Preserving embryos for use at a later time
There are many reasons to freeze embryos when undergoing infertility treatment, the most common are:
1.) Preserving surplus embryos (not used for embryo transfer)
2.) Freezing all embryos in cases of:
- a risk of hyperstimulation syndrome
- an inadequate growth of uterine lining
- medical finding on the reproductive organs which prevents the creation of conditions necessary for embryo implantation
- other acute diseases that are incompatible with pregnancy
12Extended embryo culture to blastocyst stage
Extended embryo culture is a term used to describe the embryo remaining in a culture medium from the third day after fertilization onwards. Its advantage is that is provides the possibility to judge the embryo’s deve¬lopment and morphological characteristics.
Permanent monitoring of the embryo’s deve¬lopment provides us with much more information about the dynamics of cell division and very often the healthiest embryos suitable for transfer are known already at day 2 of culturing.
Culture duration is also influenced by the following factors:
1.) endometrial development (growth of uterine lining)
2.) possible occurrence of hyperstimulation syndrome
3.) time necessary for genetic screening of biopsy cells during PGD/PGS
- Ultrasound control of the uterus, endometrium and ovaries.
- Swabs that reveal specific infections (Chlamydia, Mycoplasma, Ureaplasma)
- Preventive cytological Pap test, cervical smear
- Mamography for women over 40 years
- General health assessment (EKG, blood test, liver tests, urine control, heart and lungs control with the internist’s conclusion that you are healthy and there are no serious chronic diseases or the familiar tendency to thrombosis)
- For husband/partner, we need to know the results of sperm analysis
- Both partners should know their blood group and Rh factor
Frequently asked questions
How many days do I have to stay in Prague?
There are two options regarding number of visits to Prague:
come once, stay in Prague 5-7 days, this is how long it takes from donor´s ovarian pick up, fertilization by PICSI, monitoring of embryo development outside of a body, embryo transfer + one day of rest after the transfer. In this case, first step would be to open your file, I am sending you attached questionere with general data we would need to know about you.
come twice, once for 1st consultations and sperm cryopreservation (1 day in Prague) and 2nd time for embryo transfer (2-3 days in Prague)
Who are the donors?
In egg donation program we only use eggs from healthy young donors who undergo hormone screening, an ultrasound examination, psychological screening and repeated tests for sexually transmitted diseases (HIV, HBsAg, HCV, syphilis). They also undergo karyotype screening and genetic screening to reveal the presence of CFTR gene mutations which cause cystic fibrosis. Our donors are Czech women, 18-34 years, usually university students or young mothers on maternity leave. None of them are treated for infertility and egg donation is their free choice. Czech law states that egg donation is anonymous for both, donor and recipient.
We have a database with active donors, who are then matched to the recipient based on blood groups (both partners) and physical characteristics (eye colour, hair colour, height, weight).